Phytochemical analysis and antibacterial evaluation of ethanol stem bark extract of Azadirachta indica Grown in Dibila Kebelle, Tigray-Ethiopia

  Mengisteab Gebrehiwot Meressa

Abstract-Azadirachta indica is member of the Meliaceae family and is popularly known as margosa tree. The aim of this study was to investigate the phytochemical composition and the antibacterial property of the medicinal plant, Azadirachta indica. For this purpose, the air dried powdered stem bark sample (400 g) of the plant was extracted by soaking in 1000ml of with ethanol for 72 hours and concentrated using rotary evaporator. The antibacterial activities of these extracts were investigated against gram positive bacteria (S. b hemolytic and Staph. aureus) and gram negative bacteria (Salmonella typhi and Escherichia Coli).The antibacterial activity was tested using Muller Hinton Agar medium by disc diffusion method and minimum inhibitory concentration assays. After incubation, zone of inhibition was measured in mm, a good inhibition (>9mm) was observed indicating the effective antibacterial activity of the bioactive compounds in the plant extract. This research work also demonstrated that the presence of Phytoconstituents such as Tannins, Saponins, Phlobatanins, Flavanoids, Cardiac glycosides and Alkaloids in the stem bark ethanol crude extract of Azadirachta indica.

Keywords- Keywords-Azadirachta indica, Meliaceae, medicinal plant, Antibacterial, phytochemical, Staphylococcus, Escherichia coli aureus, Muller Hinton Agar medium.

Unique Identification Number - IJEDR1704182
Page Number(s) - 1129-1132
Pubished in - Volume 5 | Issue 4 | December 2017
DOI (Digital Object Identifier) -   
Publisher - IJEDR (ISSN - 2321-9939)

  Mengisteab Gebrehiwot Meressa,   "Phytochemical analysis and antibacterial evaluation of ethanol stem bark extract of Azadirachta indica Grown in Dibila Kebelle, Tigray-Ethiopia", International Journal of Engineering Development and Research (IJEDR), ISSN:2321-9939, Volume.5, Issue 4, pp.1129-1132, December 2017, Available at :http://www.ijedr.org/papers/IJEDR1704182.pdf