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Paper Details
Paper Title
Determination and Partial Purification of Pectinase Enzyme Produced by Aspergillus Niger using Pineapple Peels as Sole Source of Carbon
Authors
B.Leelamani, Dr.D.Sailaja, Dr.K.Kamala
Abstract
The agricultural wastes generated from Pineapple (Ananas cosmosus) represents about 35% of the entire fruit. These wastes can be converted to most useful products such as pectin. Pectin was extracted from Pineapple Peels with a percentage yield of 8.33% at pH 2.2 and temperature of 70°C. The Aspergillus niger was isolated from soil containing decomposing Pineapple Peels and was induced to produce extracellular pectinase in submerged fermentation using pectin extracts from Pineapple Peels. Then from the extracted enzyme protein concentration was determined and partially purified by ammonium sulphate precipitation and dialysis method.
Keywords- Pineapple Peels, Aspergillus niger and Dialysis.
Publication Details
Unique Identification Number - IJEDR1801120Page Number(s) - 696-700Pubished in - Volume 6 | Issue 1 | March 2018DOI (Digital Object Identifier) - Publisher - IJEDR (ISSN - 2321-9939)
Cite this Article
B.Leelamani, Dr.D.Sailaja, Dr.K.Kamala, "Determination and Partial Purification of Pectinase Enzyme Produced by Aspergillus Niger using Pineapple Peels as Sole Source of Carbon", International Journal of Engineering Development and Research (IJEDR), ISSN:2321-9939, Volume.6, Issue 1, pp.696-700, March 2018, Available at :http://www.ijedr.org/papers/IJEDR1801120.pdf
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