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ISSN: 2321-9939 | ESTD Year: 2013

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Paper Title
Purification and characterization of the antibiotic metabolite and testing for antimicrobial potential
  Tirupathi Rao A,  Srinivas N,  Veerendra Kumar B

Bacteria isolated from marine sediment has been evaluated for its bioactivity which showed broad spectrum antimicrobial activity against pathogenic microorganisms. Bioactive compound producing microbes has been identified by agar diffusion method. Secondary metabolite production of the microbe, the bioactive compound has been separated through chromatographic techniques. Column chromatography was used to isolate the bioactive compound, which was characterized to be by various spectroscopic techniques such NMR, IR and LCMS and it was found to be apparently bioactive compound 2-[2-Amino-5-(formyl-hydroxy-amino)-pentanoylamino]-5-({2-[2-amino-5-(formyl-hydroxy amino)-pentanoylamino]-3-hydroxy-butyryl}-hydroxy-amino) pentanoic acid. Minimum Inhibitory Concentration (MIC) of the active compound ranged from 0.5–15 µg/mL which was found to be comparable with the standard antibiotics. Viable cell count studies of the active compound showed it to be bactericidal in nature. Further, the compound tested for its biosafety, it was found neither to be cytotoxic nor mutagenic. Cytotoxicity studies of the compound on cancer cell lines showed a valuable cytotoxic potential against all tested cancer cell lines. The current study demonstrated that the bioactive compound isolated from Staphylococcus sp. seems to be a stable and potent antimicrobial.

Keywords- Purification, Staphylococcus epidermidis, Chromatographic analysis, Antibiotics, Biochemical Techniques
Publication Details
Unique Identification Number - IJEDR2101027
Page Number(s) - 203-209
Pubished in - Volume 9 | Issue 1 | January 2021
DOI (Digital Object Identifier) -   
Publisher - IJEDR (ISSN - 2321-9939)
Cite this Article
  Tirupathi Rao A,  Srinivas N,  Veerendra Kumar B,   "Purification and characterization of the antibiotic metabolite and testing for antimicrobial potential", International Journal of Engineering Development and Research (IJEDR), ISSN:2321-9939, Volume.9, Issue 1, pp.203-209, January 2021, Available at :http://www.ijedr.org/papers/IJEDR2101027.pdf
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