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Paper Details
Paper Title
Optimization of Downstream Process Parameters for Isolation of Androstenedione Using Statistical Approach
Authors
Umesh Luthra, R. R. Patil, Nishtha Singh
Abstract
4-Androstenedione (AD), a 19-carbon steroid hormone is an oxidation product of phytosterol fermentation by Mycobacterium sp.The isolation of the product from the fermentation broth is a challenging task. Downstream processing is an important aspect of all biotechnological processes and has significant implications on quality and yield of the final product. In the present study, the statistical approach was employed to optimize the various parameters for the downstream processing of AD. The quantity of Hyflow (filtration aid) was optimized as 5%, initially using the one variable at a time method. Further the factors: rpm, volume, solvent polarity and extraction temperature were studied using full factorial design. Analysis of the experiment showed that the polarity and the extraction temperature had maximum impact on the AD extraction. Selection of solvent for extraction procedure was carried out using one variable at a time (OVAT) technique. Ethyl acetate showed maximum product recovery. A Central Composite Design of “response surface methodology” was conducted to optimize the most significant factors of downstream process, to maximize the product extraction. The optimized model shows rpm-3500, solvent volume-5V and temperature-30°Cgives maximum extraction of androstenedione.
Keywords- Androstenedione, downstream processing, Hyflow, solvent extraction, full factorial design, response surface methodology.
Publication Details
Unique Identification Number - IJEDR1601106Page Number(s) - 618-625Pubished in - Volume 4 | Issue 1 | March 2016DOI (Digital Object Identifier) - Publisher - IJEDR (ISSN - 2321-9939)
Cite this Article
Umesh Luthra, R. R. Patil, Nishtha Singh, "Optimization of Downstream Process Parameters for Isolation of Androstenedione Using Statistical Approach", International Journal of Engineering Development and Research (IJEDR), ISSN:2321-9939, Volume.4, Issue 1, pp.618-625, March 2016, Available at :http://www.ijedr.org/papers/IJEDR1601106.pdf
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